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Discovery of a Potent and Orally Bioavailable Hypoxia-Inducible Factor 2alpha (Hif-2alpha) Agonist and its Synergistic Therapy with Prolyl Hydroxylase Inhibitors for the Treatment of Renal Anemia

Trends Immunol 34(3):144150

To do that, the URA3 open reading frame (ORF) was amplified from plasmid pCM190 (Euroscarf), GFP fused to ADH1 terminator sequence ( GFP-tADH1 ) and HIS3MX6 sequence were amplified from plasmid pFA6a-GFP(S65T)-His3MX6 [32], the TEF1 promoter ( pTEF1