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Characterization of OppA binding efficacy to cancer cells To examine the binding efficacy of the OppA proteins to heparan sulfate on cancer cells, we recombinantly expressed the OppA proteins (Supplementary Table 3) with a cMyc tag fused to the C terminus for detection (Supplementary Fig

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Protein expression was induced with 1 mM Isopropyl b-D-1-thiogalactopyranoside (IPTG) at 16C for 8 h

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doi: 10.1038/nrclinonc.2012.194 182 SunTWuHLiYHuangYYaoLChenXet al