Together, these results support the view that neutrophils are important targets for the diagnosis and therapy of RA
However, lower concentrations (typically ~ 10-50 mM) of imidazole are used in the binding and wash buffers to prevent proteins with a few opportune histidine residues from binding to the nickel column (Hemdan et al., 1989
doi: 10.1021/acsami.9b09784 136 WilhelmSMAdnaneLNewellPVillanuevaALlovetJMLynchM
Absolute pressure varies as a function of elevation

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