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Gastroenterology 1982, 82: 346-357

For subcutaneous injections, the specific site (e.g., abdomen vs

After washing away free PARP1 and NAD + , 1 g Sororin was added and incubated at 4 C for another 1 h, then the bound proteins were washed three times and boiled for 10 min at 95 C before immunoblotting with the indicated antibodies