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Cell culture HOXC8-imESCs, Control-imESCs, and parental cell line imESCs were maintained by 10 cm collagen-coated culture dishes (Corning Life Science, Tewksbury, MA) in DMEM/F12 medium (Wako, Osaka, Japan), supplemented with 10% FBS (Gibco, Rockville, MD), 2 mM L-glutamine (Gibco), and a 1% antibiotic-antimycotic solution (Wako), at 37 C in a humidified atmosphere of 5% CO and 95% air

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If the folate trap is avoided, we may not have signs of B12 deficiency anemia

Methods: To investigate the role of endothelial cell HSPA12B (eHSPA12B) in sepsis or bacterial infection, we induced polymicrobial sepsis or bacterial infection (E.coli-GFP, 5x10 / mice) in wild-type and eHSPA12B -/- mice

This area of research warrants further exploration, and these results must be taken cautiously as the associations were weak and there were no group differences in metabolites at 12 days or 6 months