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The supernatant was removed, and cells resuspended in 1 mL of Homogenate buffer (350 mM Tris-HCl, pH 7.8, 250 mM NaCl, 50 mM MgCl 2 ) supplemented with 1 mM phenylmethylsulfonyl fluoride [PMSF

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Cancer cells lacking blood circulation expanded 12 mm 3 in diameter and afterward halted, but when put in a location where angiogenesis was feasible, they expanded to more than 2 mm 3

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