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When fully dissociated, cells were centrifuged (230 g , 3 min) and the cell pellet resuspended and diluted to approximately 2.5E06 cells/mL in Recovery TM Cell Culture Freezing Medium (Gibco, #12648-010)

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Par et al

Finally, the tube lengths from two wells are calculated and averaged using ImageJ software (National Institutes of Health, Bethesda, Maryland, United States)

During a live study on gastric cancer (GC), the secretion of HGF by CAFs enhanced the presence of interleukin-6 receptor (IL-6R) in MET non-amplified GC cells via the HGF/c-MET/ERK1/2 signaling pathway [49]