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Variability in mucociliary clearance speed and mucus thickness, often affected by patient conditions such as anatomical differences, inflammation or allergies, and even the technique of administration (angle of spray, depth of insertion) (Riese et al., 2014), can alter drug absorption and uptake into the brain

Plaza-Diaz J, Solis-Urra P, Aragon-Vela J, Rodriguez-Rodriguez F, Olivares-Arancibia J, Alvarez-Mercado AI

G9260, G9261, G9262 ApoLive-Glo Multiplex Assay Measure viability and caspase-3/7 activity in the same sample well to confirm mechanisms of cell death

Male Sprague-Dawley rats were used for behavioral testing, with 24-month-old rats of mixed sex also included for aged rat models

This can occur even with perfect technique because the capillary network is invisible from the surface