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Aliquots at the various time points after fecal slurry inoculation were collected and acidified with 2 mL 0.1N HCl (Sigma-Aldrich, 50%/50% v/v), centrifuged at 4 C at 900 g for 8 min, filter-sterilized in a 0.22-m cellulose acetate filter, and frozen at 80 C until performing the experiments for the determination of the amount of TMA in the fecal slurry supernatants

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Nephrology 11 , 226231 (2006)

However, 22%25% of patients are recurrent after resection in 10 years, and 50%70% are recurrent after transplantation (Vibert et al., 2020)

Through this process, curcumin and its phase 1 metabolites are further metabolized, forming glucuronide and sulfate conjugates, making it bulkier and more water soluble