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vi) diastereomer separationsa technique whereby a racemic compound is reacted with an enantiomerically pure reagent (the chiral auxiliary) that converts the individual enantiomers to diastereomers

Constructs were induced with IPTG, lysed through microfluidics, and purified using a Bio-Rad NGC chromatography system and Glutathione Sepharose columns Proteins were and screened via SDS- PAGE and quantified via BCA assay before being utilized in recombinant protein ELISA

Histological analysis of the main organs was performed via H&E staining

The flocculent precipitate in solution is removed for centrifugation, and the centrifuged solid is then re-dissolved in deionized water

38 , doi:10.1146/annurev.biophys.050708.133655 (2008)