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All volunteers were found to be healthy based on their medical history and physical and clinical laboratory examination including serology, hematology, and biochemistry tests

Telomere length estimation was performed using the qPCR technique (OCallaghan and Fenech 2011)

Dissection of organs from the adult zebrafish

Therefore, the more hydrogen ions present, the lower the pH

Limitations include the fact that both folate and B 12 were only measured at baseline, we did not have other biochemical measures of these micronutrients (which limits accuracy in determining blood status) and we lacked data pertaining to dietary intake of these vitamins