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For urine samples, 15 l of a sample (calibration samples, QCs or PK samples) were extracted with 35 l of a 50:50 mixture of acetonitrile and methanol containing 12.5 ng ml 1 of caffeine as internal standard for 15 min at 2,000 rpm on an Eppendorf MixMate vortex mixer

10.1042/bj0540689 47 HuW.SongH.Sae HerA.BakD

Tian L, Huang C, Fu W, Gao L, Mi N, Bai M, et al

For these reasons, exogenous provision of GSH is impractical and non-physiological, while GlyNAC supplementation corrects GSH deficiency and lowers OxS in vital organs, and extends life

For example, HBx inhibits p53 activity, leading to the upregulation of lncRNA-HUR1 expression, which promotes cell proliferation and cancer progression (40)