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The protein concentration was determined using the BCA Protein Assay Kit (Pioneer, Shanghai, China), and then the protein samples were transferred onto PVDF membranes by SDS-PAGE electrophoresis and then incubated with specific primary antibodies at 4 C overnight

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Biochem 40:90409048 Bode S, Quentmeier CC, Liao PN, Hafi N, Barros T, Wilk L, Bittner F, Walla PJ (2009) On the regulation of photosynthesis by excitonic interactions between carotenoids and chlorophylls PNAS 106:1231112316 Bolwell GP, Blee KA, Butt VS, Davies DR, Gardner SL, Gerrish C, Minibayeva F, Rowntree EG, Wojtaszek P (1999) Recent advances in understanding the origin of the apoplastic oxidative burst in plant cells

Diabetes 52 , 21292136 (2003)

Although the injections produced quicker results, they may have a higher risk of adverse effects, including possible allergic reactions or injection-related issues, and require continuous treatments to maintain brightness