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Equal amounts of proteins from lysates were separated by SDSPAGE using Mini-PROTEAN TGX 10% Precast Gels (Bio-Rad, Hercules, CA, USA) before transfer onto a Sequi-Blot PVDF membrane (Bio-Rad) using the Mini Trans-Blot Electrophoretic Transfer Cell system (Bio-Rad)

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Each compound is always drawn separately and injected separately, even when both are administered in the same session