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In BV2 microglial cell lines subjected to lipopolysaccharide (LPS)-induced neuroinflammation, both lactylation-mediated inhibition of p53 activity and direct knockdown of p53 protein can disrupt the control of the NF-B inflammatory signaling pathway, ultimately leading to abnormal expression of pro-inflammatory factors and M1 polarization of microglia [132]

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More laborious methods that add UV-crosslinking, such as photo-crosslinking-assisted m 6 A-sequencing (PA-m 6 A-Seq) 27 and m 6 A individual-nucleotide resolution UV crosslinking and immunoprecipitation (miCLIP) 28 permit detection of m 6 A modifications at higher resolution

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Nature 191 , 728730 (1961)