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Air-conditioned room temperature (20-22C) causes less damage than summer heat (30C+)

Wolfe GI, Ward ES, de Haard H, Ulrichts P, Mozaffar T, Pasnoor M, et al

The inclusion of 0.9% benzyl alcohol provides antimicrobial preservation, helping maintain solution integrity following repeated vial access when appropriate aseptic laboratory techniques are utilized

An intravenous infusion is defined as the delivery of fluids through a vein using a needle or similar device

Each coverslip was washed three times with PBS, blocked with blocking solution (1x PBS, 10 % normal goat serum (NGS) and 0.3 % Triton X-100) for 30 min at RT and incubated with primary antibodies (anti-GFP (rabbit polyclonal, used at 1:1000 in IF, Abcam, Cat# ab6556, RRID:AB_305564), anti vGLUT1 (guinea pig polyclonal, used at 1:500 in IF, Synaptic Systems, Cat# 135 304, RRID:AB_887878)) diluted in blocking solution for 2 h at RT