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As the global fight over smokers moves from the United States and other countries where tobacco consumption is on the decline, Big Tobacco has drawn a line around developing nations that account for an increasingly important share of their revenues

The reaction was preincubated with fluorescence monitoring (ex/em 485/580 nm) at 37 C in a FluoStar Omega plate reader (BMG Labtech) for 15 min before addition of Crn4