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After deparaffinization, sections were heated in an autoclave at 121 C for 5 min in 10 mM sodium citrate buffer (pH 6.0) or reacted with protease solution (Nichirei Biosciences, Tokyo, Japan) for epitope retrieval, after which endogenous peroxidase was inactivated by 0.3% hydrogen peroxide in methanol

This pathway safely degrades homocysteinea potentially toxic byproduct of metabolisminto cysteine through two sequential enzymatic steps involving cystathionine beta-synthase (CBS) and cystathionine gamma-lyase (CSE)

HCR is an efficient, enzyme-free amplification strategy that isothermally produces nicked double-stranded DNA with periodically repeated modules via the assembly of two DNA hairpins, triggered by a DNA initiator

J., Zhang, H., & Rinna, A

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