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The significantly higher endometrial levels of proinflammatory cytokines (IL-6, TNF-) in the model group are consistent with a pathogenic cascade that includes dysbiosis of the gut microbiota, LPS translocation, and activation of the TLR4/NF-B signaling pathway ( n = 3) precluded balanced distribution across estrous cycle stages, rendering cycle confounding a potential source of bias in estimating LT effects that necessitates cycle-stratified validation