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doi: 10.1093/carcin/bgw102 49 KanagarajK.Abdul Syed BasheerudeenS.Tamizh SelvanG.JoseM

ADH2 is used by the yeast to convert ethanol back into acetaldehyde, and it is expressed only when sugar concentration is low

To assess phosphorylation in vitro, substrates GST-G6PD WT, GST-2YF or GST-2YA (1.5 g) was incubated with recombinant SRC (0.5 g) for 30 min at 37 C in a 50 l kinase reaction system (50 mM Tris-HCl pH 8.0, 150 mM NaCl, 100 mM DTT, 10 mM MgCl 2 ) with 0.3 mM ATP