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47 The dysregulated Wnt/-catenin pathway contributes to diabetic kidney disease (DKD)-related renal dysfunction through multifaceted mechanisms: In podocytes, chronic activation induces slit diaphragm protein (nephrin, podocin) downregulation via Snail1-mediated repression and promotes apoptosis through Bim upregulation, exacerbating albuminuria (podocyte-specific -catenin knockout reduces albuminuria by 40% in diabetic models)

[DOI] [PubMed] [Google Scholar] Murakami K, Sugimoto T, Woo M, Nishida N, Muro H

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Abnormal parasympathetic responses to stress exacerbate hypermobility and Ehlers-Danlos syndrome symptoms

Beads were washed 3x in lysis buffer, 2x in lysis buffer with 500 mM NaCl, 2x in wash buffer (10 mM TrisCl pH 8.0, 0.25 M LiCl, 1 mM EDTA, 0.5% NP-40, 0.5% Na-deoxycholate), and 2x in TE pH 8.0