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Glucose levels were standardized to protein levels, and then used to graph over three replicate experiments using GraphPad Prism 8.0 ( RNA extraction and RT-qPCR Approximately 200 synchronized L4 stage animals were transferred to treatment plates and grown at 20 C for 6 days, then washed off the plates with M9 buffer and rinsed twice with DEPC-treated water

Regen Ther 26:478488 Umer A, Ahmad K, Khan N, Greene DL, Shamim S, Habiba UE (2024b) Meta-analysis highlight the therapeutic potential of stem cells for premature ovarian failure

[DOI] [PMC free article] [PubMed] [Google Scholar] 235.Ruehle MA, Li MTA, Cheng A, Krishnan L, Willett NJ, Guldberg RE

Moreover, research has demonstrated that cobalamin is not only essential to this process but also actively promotes the expression and synthesis of the components necessary for the formation and repair of the myelin sheath [76]

Transfection was carried out for 24 h with Lipofectamine 3000 (L3000075, Invitrogen, USA) and P3000 (L3000075, Invitrogen, USA) transfection reagents mixed with OPTI-MEM (Gibco, Shanghai, China)