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Study Design There were three experimental groups for this study (Figure 1): High intensity stimulus (HI, n=5), low intensity stimulus (LI, n=5), and nave unstimulated control (NC, n=5)

Regular medical monitoring is recommended to manage these side effects and ensure the therapys effectiveness without adverse reactions

Kinetic analysis of drug release The rate at which the drugs were released from the tested mixtures was studied using the linear regression analysis method (R 2 )

26,29,30,31 Acute precipitated withdrawal In Experiment 1, to establish the model and validate the behavioral scoring, pups were randomized within litter to receive either naloxone hydrochloride (1 mg/kg, intraperitoneal (i.p.)), a dose that has been shown to precipitate acute withdrawal in rats, 26,29,30,31 or an equal volume of saline

Standard dosing protocol: Dose: 250-500mcg per injection Frequency: Twice daily (morning and evening) Duration: 4-8 weeks depending on injury severity Injection site: As close to injury as safely possible Weight-based dosing: Some people dose by body weight: 2.5-4 mcg per kilogram