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Oocytes were washed four times with DPBS and incubated in staining working solution containing 3 M Nile Red (MCE, Shanghai, China) in the dark for 30 min at room temperature

For comparisons between 2 groups, 2-tailed Students t test for unpaired or paired data was used

Animal studies have shown that increasing sRAGE levels can prevent RAGE-mediated dysfunctions and even reverse some detrimental RAGE-related consequences (157161)

Beyond-Use Date (BUD): Assigned by compounding pharmacies for compounded formulations

Lion Pose's formulation includes all these elements in a triple lipid complex with ceramides, cholesterol, and fatty acids that mimic the skin's natural structure