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The following primary antibodies were used: anti-ATP7A (Santa Cruz Biotechnologies, Texas, USA, sc-376467), anti-CTR1 (abcam, Massachusetts, USA, #ab129067), anti-ABCG2 (Cell Signaling Technology, Massachusetts, USA, #42078), and -actin as a loading control (Cell Signaling Technology, Massachusetts, USA, #8457)

These findings are consistent with evidence from the literature and with WHO guidelines, which warn that novel tobacco products emit harmful chemicals that can cause adverse effects 1,13-16

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In Australia, 25 cigarettes per pack are common

A burndown herbicide that kills all of the plants at once can disrupt the food supply to soil microbes