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Frontiers in Molecular Neuroscience 8 , 46, (2015)

Growth conditions Strains were grown at 37C in either M9 medium or minimal A medium (Miller, 1972) containing 0.2% glucose, 0.2% casamino acids, 0.5 mM histidine, 0.5 mM tryptophan, 0.5 mM thiamine, 0.01% MgSO 4 , and 0.01% CaCl 2 , unless otherwise noted

The main trigger of articular bone erosion is synovitis, including the overproduction of ROS and proinflammatory cytokines, as well as RANKL, thereby creating a highly inflammatory and tissue-damaging microenvironment of RA