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Blood tests typically include HbA1c or fasting glucose (to screen for diabetes), lipid profile, and morning total testosterone levels if hypogonadism is suspected (with repeat testing and measurement of luteinising hormone [LH] and follicle-stimulating hormone [FSH] if testosterone is low)

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Rectal tumour biopsies ( n =10) were collected from consenting patients undergoing diagnostic endoscopy, a baseline measurement of metabolic rate was recorded, biopsies were treated for 24 h with metformin (10 mM) or H 2 O vehicle control and metabolism was assessed at 24 h post treatment

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