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Rats were acclimatized to a controlled environment of 22 1 C temperature and a 12-h light/dark cycle, with free access to food and water, for 2 weeks prior to the experiments

Michel, J.-B., Lagrange, J., Regnault, V

O-Acetyl Serine Determination Metabolite profiling of milkripe seed tissue by gas chromatographymass spectrometry (GCMS) was performed essentially as previously described ( 13 C 6 -sorbitol at 17 mg/L was added to the methanol extraction step to allow later correction of analytical variance, and 160 l, rather than 100 l, polar phase aliquots were taken for analysis

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