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Cells were dissociated using 5 mM EDTA in HBSS and resuspended in medium with B27 supplement for 4 h before incubation with recombinant BDNF protein (100 nM, Peprotech, 450-02) or vehicle (equal volume of 0.1% BSA in H 2 O)

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Permutation test has been shown in Figure 5(b), Q2 was less than R2 and Q2 intersects the Y-axis negatively, proving that the model was applicable