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Buffer A: 50 mM Tris-Cl pH 7.5, 300 mM NaCl, 1% SDS, 0.2 mM PMSF, 10 mM NEM, 20 M MG-132, Benzonase, 20 mM MgCl 2 , complete protease inhibitor Structure modeling For modeling of Ube3C critical residues within its structure, we used AlphaFold 3 74

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