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Lets say that your target protein is smaller than GST, such that most of the size of the GST-fusion protein will be contributed from GST and not your target protein

A community-based RCT by Flora et al

doi: 10.3390/nu13061848 6 ArentzSSmithCAAbbottJBensoussanA

10.3969/j.issn.1003-9198.2023.04.007 212 WangL.SuQ

This suggests that selective FAA transport occurs in the mammary gland, that FAA levels in human milk are strictly regulated and, consequently, that FAAs are likely to be of physiological relevance in the developing infant