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The fact that the overall similarity of these orphan sequences with GSTLs is low and that they do not necessarily form a single clade with GSTLs could come from their rapid and independent evolution

The source of irradiation was a 15 watt lamp emitting UV light with a wavelength of 312 nm (UVP, Upland, CA, USA)

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To examine the accuracy of transcript-level expression profile, the gene-level profile of 6 DEGs which linking pco105094 and umc2770 with qRT-PCR were compared to those of DEGs with RNA-seq

Moreover, this speculation was also confirmed by further EMSA experiment that SlBEL11 directly binds to the promoter of SlLCY-b2 containing TTGACTTGAC atagt GTCA element (Figure 3)