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The inability of 5-cholesten-3-one to serve as substrate for the bacterial ketosteroid isomerase was noted in the original publication (Talalay and Wang, 1955), and the purified mammalian GST A3-3 from different sources also lack detectable activity with this steroid (unpublished work in our laboratory)

Acta Anaesth Scand 60(5):623633 Nogueiras R, Romero-Pic A, Vazquez MJ et al (2012) The opioid system and food intake: homeostatic and hedonic mechanisms

Cells were aliquoted and stored at 180 C until use

Diagnosis of myalgic encephalomyelitis/chronic fatigue syndrome with partial least squares discriminant analysis: relevance of blood extracellular vesicles

Endpoints The primary endpoint of the study was the incidence of migraine aura within the 12-hour observational period following the initiation of CGRP infusion