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Cell viability was determined by mixing an aliquot of the suspension 1:2 with trypan blue and counting viable cells using a hemocytometer

Many immunomodulatory factors are found in the tegument, the multi-layered outer surface of the parasite which is capable of quick self-renewal after damage, a process mediated by a population of somatic stem cells with a propensity to differentiate into tegument associated cells (147).The factors within the tegument have been extensively examined (148, 149)

After 24 h of incubation, spheroids were collected from the microwells using cut 1000 L pipette tip and transferred into a sterile 10-cm Petri dishes in cortical differentiation medium [E6F with 10 M dorsomorphin (DM

Synergistic Natural Products in Anti-Ageing: Mechanistic Insights, Experimental Evidence, and Translational Perspectives Yuanze Sun 1,2 Lihong Sun 2 Johnson Liu 3 Ji He 4 Yu Feng 4 Jun Lu 1,4,5,* Received: 18 November 2025 Revised: 13 January 2026 Accepted: 20 January 2026 Published: 26 January 2026 2026 The authors