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DNA Protection and Epigenetics: How Epitalon May Slow Down the Aging Clock While telomeres mark the visible end of the cellular lifespan, the epigenome serves as its regulatory backbone

Bacterial cells were harvested by carefully removing the supernatant by centrifugation (1,000 g , 10 min, 4 C) and the resulting pellet was resuspended with 50 l of B-PER reagent (supplemented with 1 mM DTT and 1 mM PMSF)