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2 Materials and methods 2.1 Experimental mouse models In the acute SCI model, adult male C57BL/6N mice (1012 weeks, male, Nihon SLC, Shizuoka, Japan) were subjected to contusion injury at T9T10 using an Infinite Horizon Impactor (60 kdyn) or sham-operated by laminectomy only, as described previously (Figure 1A) (Scheff et al., 2003)

Perez-Jimenez F, et al

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Among peripheral organs, GABA is abundant in the pancreas, and recent research suggests that the pancreatic GABA system plays an important role in protecting the pancreas and regulating insulin metabolism 22

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