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In another pilot-scale phase I clinical trial using ADI-PEG20 with docetaxel on different solid tumors like non-small cell lung cancer, castrate resistant prostate cancer and head and neck squamous cell carcinoma [142]

While some polynitrosylated proteins, like bovine serum albumin (BSA), exhibited physiologic nitric oxide properties such as vasodilator and anti-platelet activities, nitrosylation of enzymes generally had an adverse effect on enzymatic activities

However, IR can also promote the secretion of transforming growth factor (TGF-), inhibit CD8 + T cells and increase the proportion of regulatory T cells (Tregs), leading to immunosuppression in tumors