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Briefly, independent cultures of Agrobacterium carrying pTRV1 or pTRV2-based constructs were resuspended in 10 mM MgCl 2 , 10 mM MES (pH 5.6) and 150 M acetosyringone at OD 600 = 0.8, and incubated for 2 h at 28 C in the dark

This dual action of inhibiting inflammatory drivers while boosting endogenous antioxidant defenses makes the combination of curcumin and EGCG highly relevant for patients managing the complex immune dysregulation of Long COVID

Within the first week, the stiffness disappeared

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Together, these findings suggest that cellular resistance to cuproptosis also depends on a hierarchically organized copper-buffering system, in which mtGSH provides rapid, dynamic metal buffering, whereas metallothioneins confer sustained high-affinity sequestration to prevent mitochondrial copper overload