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Comprehensive toxicological evaluations should include assessments of hematological, hepatic, and renal function, immunotoxicity (e.g., complement activation, cytokine release), and histopathological analysis of major organswith special attention to carrier-related risks such as NP accumulation in the reticuloendothelial system and chronic inflammatory responses

enterocytes), as well as for the phagocytic activity of macrophages and production of GSH, which is the most potent antioxidant in the body [20, 33, 35]

Andrology Center and American Center for Reproductive Medicine, Cleveland Clinic, Mail Code X-11, 10681 Carnegie Avenue, Cleveland, OH 44195, USA

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HIF-, levels of mitochondrial DNA, and other mitochondrial DAMP molecules may also serve as useful biomarker and need to be investigated