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The MsGSTU17 gene was found to be located in the cytoplasm and had high expression levels in both leaves and roots

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Notably, lignans exhibit anti-cancer activity through multiple regulatory pathways ( 3 Factors affecting the appropriate extraction methods for phenolic compounds Polyphenols comprise a broad array of chemical structures, resulting in varied chemical and physical properties ( Furthermore, extraction efficiency is highly sensitive to operational parameters such as pH, type of solvent employed, solvent-to-sample ratio, and duration of the extraction process ( 3.1 Extraction methods of phenolic compounds 3.1.1 Ultrasound-assisted extraction method Ultrasound-assisted extraction is a widely used and efficient technique for isolating phenolic compounds, offering high yields in a relatively short time ( The process relies on acoustic cavitation, wherein ultrasonic waves disrupt plant cell walls by inducing rapid expansion and contraction of solid surfaces ( Randia monantha , mango seed kernels, olive pomace, and pine needles ( Ultrasound-assisted extraction provides notable advantages for the extraction of polyphenols

In terms of diagnosis, the detection of fecal butyric acid concentration or the plasma SCFAs profile is expected to become an objective biomarker for evaluating the disease severity and predicting the 28-day mortality, providing a new basis for early risk stratification (33)

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