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Western blot Worm Lysate from synchronous L3 worm cultures was prepared in Laemmli buffer with 6 M urea with glass beads in a FastPrep machine (MP Biomedicals, Irvine, CA) for 2 30 s

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Alpha-lipoic acid Increased oxidative stress caused by free radical formation and defects in antioxidant defense related to hyperglycemia is thought to cause endoneurial hypoxia and nerve dysfunction, which contribute to DPN [61]

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