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In sepsis-induced ALI, Ferulic acid has demonstrated the potential to improve alveolar epithelial barrier function by activating the NRF2/HO-1 pathway, thereby inhibiting ferroptosis in alveolar epithelial cells

10.1093/toxsci/kfg223 80 LawsS

A primer efficiency of 95105% was deemed acceptable for use

Immunoblot analysis After treatment of cells with erastin or RSL3 for different durations as indicated, the cells were collected by trypsinization and centrifugation, and then lysed on ice for 30 min in the RIPA buffer containing 1% protease inhibitor cocktail

Your body makes glutathione naturally, but levels can decline with chronic stress, poor diet, aging, alcohol use, or illness