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Description

The tips were washed twice with 200 l 0.1% FA, followed by elution of the peptides with 100 l 40% acetonitrile/0.1% FA by centrifuging for 4 min at 1,500 g

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Higher D -value indicates superior quality of processed P

This server employs BLAST to identify sequence similarities, and proteins with an Expect value (E-value) less than 1 10 5 were considered homologous ( Figure 1D