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Proteins on gel were transferred to polyvinyl difluoride (PVDF) membranes followed by blocking with 5% non-fat milk for 2 h

Comparator Selection: Full-length hGH (to demonstrate receptor-independent effects) -adrenergic agonists (to show distinct mechanism) Vehicle control 4

Sections were subsequently washed and incubated sequentially in 50% and 70% ethanol for 5 min each

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