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The thicknesses were incubated with Rabbit anti-GFP antibody (1:500) at 4C overnight and then incubated with Alexa488 (Thermo) coupled with Goat anti-rabbit secondary antibody (1:500) for 1.5 h

Slavery [edit] Planters embraced the use of slaves mainly because indentured labor became expensive

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And the poor may have less room in their budgets for the additional costs