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Samples containing identical amounts of protein were resolved using SDS-PAGE (Criterion, BioRad Laboratories), transferred to nitrocellulose, and blocked in Tris-buffered saline (TBS) containing 5% milk for one hour at room temperature

Thus, engineered CAR-T cells offer a particularly promising approach to modulate T-cell metabolism

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Tumours have therefore found means of utilising diverse mechanisms to provide themselves a supply of the amino acids that they require for malignant progression

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