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Dulbeccos modified Eagle medium (DMEM), phosphate-buffered saline (PBS), trypsin/EDTA, fetal bovine serum (FBS)-gamma-irradiated, and antibioticantimycotic solution 100X (10,000 U penicillin, 10 mg streptomycin, and 25 g amphotericin B per ml in 0.9% normal saline) were obtained from Himedia, Mumbai, India

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