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Niimura et al., The experimental value of zebrafish as an animal model for in vivo drug discovery has grown rapidly due to their ease of assessment, large clutch size, and ability to recapitulate human disease conditions (MacRae and Peterson, 2007

Studies examining asthma, COPD and CF have focused on the role of the immune system [3,4,5,6,7] and epithelial ion channels in mucus secretion [8,9,10,11,12], but the involvement of the nervous system, and in particular neuropeptide signaling, remains a field of much unknowns [13]

doi: 10.1182/blood-2013-02-486217

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This creates a long-term deterrent that extends beyond the detection capabilities available at the time of collection