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Preparation of crude membranes with V2R and G-protein coupled receptor kinase 2 (GRK2) Expi-293 cells grown in suspension were co-transfected with plasmids expressing the human V2R and the membrane targeted form of GRK2 to promote receptor phosphorylation

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The HPLC/MS system that was employed consisted of a communications bus module, pumps, an auto sampler, a diode array detector and a mass spectrometer

Briefly, cells were seeded in 96-well plates at 310 3 cells per well