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In another set of experiments, ASA treatments (5 or 10 mol/ml ASA 48 h) were performed in GSH-depleted cells (BSO treatment for 18 h) which were then treated with 10 mM NAC (dissolved in DMSO, then diluted with cell culture medium to the required concentration keeping the DMSO concentration not more than 0.1%) for 2 hr before the addition of ASA for 48 h to manipulate cellular GSH pools

This stress can cause a decrease in the production of inflammatory cytokines like TNF- and interleukins (such as IL-6, IL-10, and IL-1), ultimately leading to the death of these cells

Neurology 89 , 21572166 (2017)